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COURSE UNIT TITLECOURSE UNIT CODESEMESTERTHEORY + PRACTICE (Hour)ECTS
AFINITE TECHNIQUES IN BIOTECHNOLOGY BTE530 - 3 + 0 10

TYPE OF COURSE UNITElective Course
LEVEL OF COURSE UNITMaster's Degree With Thesis
YEAR OF STUDY-
SEMESTER-
NUMBER OF ECTS CREDITS ALLOCATED10
NAME OF LECTURER(S)-
LEARNING OUTCOMES OF THE COURSE UNIT At the end of this course, the students;
1) Learns What Affinity is
2) Learn What Affinity Chromatography Is
3) May Link Affinity and Biotechnology
4) Understanding the Connection Between Molecular Biology and Affinity Techniques can learn to use
MODE OF DELIVERY
PRE-REQUISITES OF THE COURSENo
RECOMMENDED OPTIONAL PROGRAMME COMPONENT
COURSE DEFINITION
COURSE CONTENTS
WEEKTOPICS
1st Week 1. Definition of High Affinity Chromatography, Development and use of Affinity Chromatography,
2nd Week 2. Matrix selection in High Affinity Chromatography
3rd Week 3. Ligand selection in Affinity Chromatography of the Week
4th Week 4. Affinity techniques used in molecular biology today
5th Week 5. Purification of cells by chemical Affinity chromatography
6th Week 6. Molecular suppressed polymers and their applications in biochromatography
7th Week 7. Mid-Week Exams
8th Week 8. Week Immobilized Metal ion affinity chromatography
9th Week 9. Immunaffinte chromatography of the week
10th Week 10. Dye-ligand chromatography of the week
11th Week 11. Monoliths of the Week
12th Week 12. Affinity precipitation of proteins using a metal chelate
13th Week 13. Purification of proteins by various methods of Gel Filtration and ion exchange chromatography
14th Week 14. Week Lectin Affinity Chromatography
RECOMENDED OR REQUIRED READINGAffinity Chromatography, C. Christopher R. Lowe, P. D. Peter D. G. Dean John Wiley & Sons, 1974
PLANNED LEARNING ACTIVITIES AND TEACHING METHODS
ASSESSMENT METHODS AND CRITERIA
ECTS WORKLOAD
Activities Number Hours Workload
Midterm exam18484
Preparation for Quiz11616
Individual or group work000
Preparation for Final exam12424
Course hours14342
Preparation for Midterm exam11111
Laboratory (including preparation)000
Final exam19696
Homework11212
Project11212
Total Workload297
Total Workload / 309,9
ECTS Credits of the Course10
LANGUAGE OF INSTRUCTION
WORK PLACEMENT(S)No
  

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