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COURSE UNIT TITLECOURSE UNIT CODESEMESTERTHEORY + PRACTICE (Hour)ECTS
CELL CULTURE BTE511 - 3 + 0 10

TYPE OF COURSE UNITElective Course
LEVEL OF COURSE UNITMaster's Degree With Thesis
YEAR OF STUDY-
SEMESTER-
NUMBER OF ECTS CREDITS ALLOCATED10
NAME OF LECTURER(S)-
LEARNING OUTCOMES OF THE COURSE UNIT At the end of this course, the students;
1) Learn basic animal cell culture laboratory infrastructure, sterilization and aseptic techniques, and medium types.
2) Learn fundamentals of primary, suspension and monolayer cultures and passaging, cell storage techniques, and establishment of immortal cultures.
3) Perform laboratory applications of the course content, and discuss experimental results.
MODE OF DELIVERYFace to face
PRE-REQUISITES OF THE COURSENo
RECOMMENDED OPTIONAL PROGRAMME COMPONENTNo
COURSE DEFINITION
COURSE CONTENTS
WEEKTOPICS
1st Week 1. Plant Cell Culture Laboratory
2nd Week 2. Sterilization and Aseptic Techniques in Plant Cell Culture
3rd Week 3. Sterilization of Seed and Plant
4th Week 4. Media Preparation
5th Week 5. Applications and Discussions
6th Week 6. Sub-culture
7th Week 7. Applications and Discussions
8th Week 8. MIDTERM
9th Week 9. Callus Culture
10th Week 10. Direct Organogenesis
11th Week 11. Applications and Discussions
12th Week 12. Indirect Organogenesis
13th Week 13. Applications and Discussions
14th Week 14. Presentations
15th Week
RECOMENDED OR REQUIRED READING? Culture of Animal Cells: A Manual of Basic Techniques, 6th ed. R. Ian Freshney. John Wiley & Sons, Inc., 2010.
? Plant Tissue Culture Techniques and Experiments, Roberta H. Smith, Third Edition, 2012

PLANNED LEARNING ACTIVITIES AND TEACHING METHODSLecture,Discussion,Questions/Answers,Presentation
ASSESSMENT METHODS AND CRITERIA
 QuantityPercentage(%)
Mid-term130
Practice130
Total(%)60
Contribution of In-term Studies to Overall Grade(%)60
Contribution of Final Examination to Overall Grade(%)40
Total(%)100
ECTS WORKLOAD
Activities Number Hours Workload
Midterm exam18484
Preparation for Quiz11616
Individual or group work000
Preparation for Final exam12424
Course hours14342
Preparation for Midterm exam11111
Laboratory (including preparation)000
Final exam19696
Homework11212
Project11212
Total Workload297
Total Workload / 309,9
ECTS Credits of the Course10
LANGUAGE OF INSTRUCTIONTurkish
WORK PLACEMENT(S)No
  

KEY LEARNING OUTCOMES (KLO) / MATRIX OF LEARNING OUTCOMES (LO)
LO1LO2LO3
K1  X   X   X
K2  X   X   X
K3  X   X   X
K4      X
K5      X
K6      X
K7      X
K8      X
K9      X