| TYPE OF COURSE UNIT | Compulsory Course |
| LEVEL OF COURSE UNIT | Master's Degree With Thesis |
| YEAR OF STUDY | 1 |
| SEMESTER | Second Term (Spring) |
| NUMBER OF ECTS CREDITS ALLOCATED | 10 |
| NAME OF LECTURER(S) | -
|
| LEARNING OUTCOMES OF THE COURSE UNIT |
At the end of this course, the students;
1) Learn Fundamentals of Sterilization and Bacterial Cultures.
2) Learn nucleic acid and protein isolation techniques, qualitative analysis by electrophoresis, and spectrophotometric quantitative analysis
3) Learn fundamental techniques for molecular cloning of a target gene to a bacterial cell and screening of clones.
4) Learn experimental analyses with obtained recombinant organisms.
5) Learn the expression analysis via real-time quantitative RT-PCR.
|
| MODE OF DELIVERY | Face to face |
| PRE-REQUISITES OF THE COURSE | No |
| RECOMMENDED OPTIONAL PROGRAMME COMPONENT | No |
| COURSE DEFINITION | |
| COURSE CONTENTS | | WEEK | TOPICS |
|---|
| 1st Week | 1. Fundamentals of Sterilization and Bacterial Cultures | | 2nd Week | 2. Fundamentals of Spectrophotometric Analysis | | 3rd Week | 3. DNA Isolation and Quantitation | | 4th Week | 4. Plasmid Isolation and Quantitation | | 5th Week | 5. Visualization of Nucleic Acids by Agarose Gel Electrophoresis | | 6th Week | 6. Protein Isolation and Quantitation | | 7th Week | 7. Visualization of Nucleic Acids by Polyacrylamide Gel Electrophoresis | | 8th Week | 8. Midterm | | 9th Week | 9. Polymerase Chain Reaction | | 10th Week | 10. Restriction Endonuclease Digestion | | 11th Week | 11. Vectors and Ligation | | 12th Week | 12. Competent Bacteria and Transformation | | 13th Week | 13. Screening of Clones | | 14th Week | 14. Student presentations | | 15th Week | |
|
| RECOMENDED OR REQUIRED READING | 1. Moleküler Biyolojide Kullanılan Yöntemler, Prof. Dr. Güler Temizkan, 2004.
2. Molecular Cloning: A Laboratory Manual Michael R. Green,? Joseph Sambrook (Fourth Edition), 2012.
3. Current Protocols in Molecular Biology, Wiley Online Library. |
| PLANNED LEARNING ACTIVITIES AND TEACHING METHODS | Lecture,Discussion,Questions/Answers,Experiment,Report Preparation,Presentation |
| ASSESSMENT METHODS AND CRITERIA | | | Quantity | Percentage(%) |
|---|
| Mid-term | 1 | 20 | | Assignment | 1 | 35 | | Total(%) | | 55 | | Contribution of In-term Studies to Overall Grade(%) | | 55 | | Contribution of Final Examination to Overall Grade(%) | | 45 | | Total(%) | | 100 |
|
| ECTS WORKLOAD |
| Activities |
Number |
Hours |
Workload |
| Midterm exam | 1 | 84 | 84 | | Preparation for Quiz | 1 | 16 | 16 | | Individual or group work | 0 | 0 | 0 | | Preparation for Final exam | 1 | 24 | 24 | | Course hours | 14 | 3 | 42 | | Preparation for Midterm exam | 1 | 11 | 11 | | Laboratory (including preparation) | 0 | 0 | 0 | | Final exam | 1 | 96 | 96 | | Homework | 1 | 12 | 12 | | Project | 1 | 12 | 12 | | Total Workload | | | 297 |
|---|
| Total Workload / 30 | | | 9,9 |
|---|
| ECTS Credits of the Course | | | 10 |
|---|
|
| LANGUAGE OF INSTRUCTION | Turkish |
| WORK PLACEMENT(S) | No |
| | |
