TYPE OF COURSE UNIT | Compulsory Course |
LEVEL OF COURSE UNIT | Bachelor's Degree |
YEAR OF STUDY | 3 |
SEMESTER | Sixth Term (Spring) |
NUMBER OF ECTS CREDITS ALLOCATED | 5 |
NAME OF LECTURER(S) | Assistant Professor Beyza Gökçınar Marpuç
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LEARNING OUTCOMES OF THE COURSE UNIT |
At the end of this course, the students; 1) Learn basic animal cell culture laboratory infrastructure, sterilization and aseptic techniques, and medium types. 2) Learn fundamentals of primary, suspension and monolayer cultures and passaging, and cell storage techniques. 3) Learn basic plant cell culture laboratory infrastructure, sterilization of seed and plant and medium types. 4) Learn sub-culture, direct and indirect organogenesis in plant cell culture. 5) Perform laboratory applications of the course content, and discuss experimental results.
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MODE OF DELIVERY | Face to face |
PRE-REQUISITES OF THE COURSE | No |
RECOMMENDED OPTIONAL PROGRAMME COMPONENT | None |
COURSE DEFINITION | In this course, students learn the culturing methods of animal and plant cells both theoretically and practically and have knowledge about the application areas of plant-animal tissue culture. |
COURSE CONTENTS | WEEK | TOPICS |
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1st Week | Animal Cell Culture Laboratory | 2nd Week | Sterilization and Aseptic Techniques in Cell Culture | 3rd Week | Medium Types | 4th Week | Primary Cell Culture Techniques | 5th Week | Suspension Cultures and Passage | 6th Week | Monolayer Cultures and Passage | 7th Week | Storage of Cells (Freezing and Thawing) | 8th Week | Midterm Exam | 9th Week | Plant Cell Culture Laboratory | 10th Week | Sterilization of Seed and Plant | 11th Week | Media Preparation | 12th Week | Sub-culture | 13th Week | Direct Organogenesis | 14th Week | Indirect Organogenesis |
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RECOMENDED OR REQUIRED READING | Culture of Animal Cells: A Manual of Basic Techniques, 6th ed. R. Ian Freshney. John Wiley & Sons, Inc., 2010. Plant Tissue Culture Techniques and Experiments, Roberta H. Smith, Third Edition, 2012. |
PLANNED LEARNING ACTIVITIES AND TEACHING METHODS | Lecture,Questions/Answers,Experiment,Report Preparation |
ASSESSMENT METHODS AND CRITERIA | | Quantity | Percentage(%) |
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Mid-term | 1 | 35 | Practice | 1 | 25 | Total(%) | | 60 | Contribution of In-term Studies to Overall Grade(%) | | 60 | Contribution of Final Examination to Overall Grade(%) | | 40 | Total(%) | | 100 |
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ECTS WORKLOAD |
Activities |
Number |
Hours |
Workload |
Midterm exam | 1 | 2 | 2 | Preparation for Quiz | 4 | 3 | 12 | Individual or group work | | | | Preparation for Final exam | 1 | 35 | 35 | Course hours | 13 | 2 | 26 | Preparation for Midterm exam | 1 | 30 | 30 | Laboratory (including preparation) | 13 | 2 | 26 | Final exam | 1 | 2 | 2 | Homework | 1 | 8 | 8 | Quiz | 4 | ,5 | 2 | Report writing | 1 | 10 | 10 | Total Workload | | | 153 |
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Total Workload / 30 | | | 5,1 |
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ECTS Credits of the Course | | | 5 |
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LANGUAGE OF INSTRUCTION | English |
WORK PLACEMENT(S) | No |
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