| TYPE OF COURSE UNIT | Compulsory Course |
| LEVEL OF COURSE UNIT | Bachelor's Degree |
| YEAR OF STUDY | 3 |
| SEMESTER | Fifth Term (Fall) |
| NUMBER OF ECTS CREDITS ALLOCATED | 4 |
| NAME OF LECTURER(S) | Assistant Professor Aliye Ezgi Güleç Taşkıran
|
| LEARNING OUTCOMES OF THE COURSE UNIT |
At the end of this course, the students;
1) Explain timeline of recombinant DNA technology, fundamentals of molecular cloning and vectors.
2) Distinguish transformation and transfection techniques.
3) Suggest methodologies for genome and cDNA library preparation and screening.
4) Design recombinant protein production in different cell types.
5) Comment on fundamentals of custom vector design and techniques for specific purposes.
|
| MODE OF DELIVERY | Face to face |
| PRE-REQUISITES OF THE COURSE | No |
| RECOMMENDED OPTIONAL PROGRAMME COMPONENT | None |
| COURSE DEFINITION | In this course, basic principles of molecular cloning, screening of clones, determination of target gene expression, genome and cDNA libraries, and recombinant protein production are given. |
| COURSE CONTENTS | | WEEK | TOPICS |
|---|
| 1st Week | Timeline of Recombinant DNA Technologies | | 2nd Week | Molecular Cloning: PCR I | | 3rd Week | Molecular Cloning: PCR II | | 4th Week | Blotting and Hybridization Based Techniques for Screening | | 5th Week | Modification Enzymes | | 6th Week | Vectors and Screening of Clones I | | 7th Week | Vectors and Screening of Clones II | | 8th Week | Midterm Exam | | 9th Week | Gene Transfer I | | 10th Week | Gene Transfer II | | 11th Week | Genome Libraries | | 12th Week | cDNA Libraries | | 13th Week | Recombinant Protein Production in Prokaryotes | | 14th Week | Eukaryotic Recombinant Protein Production |
|
| RECOMENDED OR REQUIRED READING | Principles of Gene Manipulation and Genomics. Sandy B. Primrose, Richard Twyman, 7th edition, 2006.
Molecular Biotechnology, Principles and Applications of Recombinant DNA. Bernard R. Glick, Jack J. Pasternak, ASM Press, Washington, D.C., 2009. |
| PLANNED LEARNING ACTIVITIES AND TEACHING METHODS | Lecture,Discussion,Questions/Answers,Presentation |
| ASSESSMENT METHODS AND CRITERIA | | | Quantity | Percentage(%) |
|---|
| Mid-term | 1 | 35 | | Assignment | 1 | 15 | | Total(%) | | 50 | | Contribution of In-term Studies to Overall Grade(%) | | 50 | | Contribution of Final Examination to Overall Grade(%) | | 50 | | Total(%) | | 100 |
|
| ECTS WORKLOAD |
| Activities |
Number |
Hours |
Workload |
| Midterm exam | 1 | 2 | 2 | | Preparation for Quiz | | | | | Individual or group work | 14 | 2 | 28 | | Preparation for Final exam | 1 | 22 | 22 | | Course hours | 13 | 3 | 39 | | Preparation for Midterm exam | 1 | 18 | 18 | | Laboratory (including preparation) | | | | | Final exam | 1 | 2 | 2 | | Homework | 1 | 15 | 15 | | Total Workload | | | 126 |
|---|
| Total Workload / 30 | | | 4,2 |
|---|
| ECTS Credits of the Course | | | 4 |
|---|
|
| LANGUAGE OF INSTRUCTION | English |
| WORK PLACEMENT(S) | No |
| | |
