TYPE OF COURSE UNIT	Elective Course
LEVEL OF COURSE UNIT	Master's Degree With Thesis
YEAR OF STUDY	-
SEMESTER	-
NUMBER OF ECTS CREDITS ALLOCATED	10
NAME OF LECTURER(S)	-
LEARNING OUTCOMES OF THE COURSE UNIT	At the end of this course, the students; 1) know the basic principles of innate and adaptive immune system. 2) have a good knowledge of the basis for the structure, production and purification of monoclonal and polyclonal antibodies. 3) have a good theoretical knowledge of methods like ELISA, immunoblotting, immunohistochemical staining techniques, direct and indirect immunofluorescence assays, flow cytometry, and immunoprecipitation assays. 4) understand how immunological principles used in industry and research. 5) gain experience in literature search, presentation and discussion of current research articles or reviews about immunological techniques.
MODE OF DELIVERY	Face to face
PRE-REQUISITES OF THE COURSE	No
RECOMMENDED OPTIONAL PROGRAMME COMPONENT	No
COURSE DEFINITION	Immunological Techniques course gives information about polyclonal and monoclonal antibody generation and purification, detection of antigens in cells, tissues, fluids and solid supports using antibodies, direct and indirect immunofluorescence assays, flow cytometry, immunoassay of antigen by ELISA, immunoblotting, immunoprecipitation of antigen complexes, chromatin immunoprecipitation (ChIP) assays and also immunohistochemical staining techniques.
COURSE CONTENTS	WEEK TOPICS 1st Week Course Introduction / Basic Principles of Immunology 2nd Week Production and Purification of Antibodies 3rd Week Current literature presentation and discussion about ?monoclonal antibody technology? 4th Week ELISA and Immunoblotting 5th Week Current literature presentation and discussion 6th Week Immunohistochemical Staining Techniques 7th Week Current literature presentation and discussion 8th Week Midterm 9th Week Immunoprecipitation Assays 10th Week Direct and Indirect Immunofluorescence Assays 11th Week Flow Cytometry 12th Week Current literature presentation and discussion 13th Week Immunological Principles Used in Industry and Research 14th Week Research article presentation and discussion
RECOMENDED OR REQUIRED READING	Immunochemistry methods: Antigen and antibody production. Immunological tests, Tamara Krstic, 2015. Immunoassay and Other Bioanalytical Techniques by Jeanette M. van Emon. Immunoassays development, applications and future trends by Richard OKennedy, Caroline Murphy. The Immunoassay Handbook. Theory and Applications of Ligand Binding, ELISA and Related Techniques by David Wild. Current research articles and reviews
PLANNED LEARNING ACTIVITIES AND TEACHING METHODS	Lecture,Discussion,Questions/Answers,Presentation,Report Preparation
ASSESSMENT METHODS AND CRITERIA	Quantity Percentage(%) Mid-term 1 25 Presentation of Article 5 40 Total(%) 65 Contribution of In-term Studies to Overall Grade(%) 65 Contribution of Final Examination to Overall Grade(%) 35 Total(%) 100
ECTS WORKLOAD	Activities Number Hours Workload Midterm exam 1 3 3 Preparation for Quiz Individual or group work Preparation for Final exam 1 25 25 Course hours 13 3 39 Preparation for Midterm exam 1 20 20 Laboratory (including preparation) Final exam Homework Article Presentation 5 43 215 Total Workload 302 Total Workload / 30 10,06 ECTS Credits of the Course 10
LANGUAGE OF INSTRUCTION	English
WORK PLACEMENT(S)	No