At the end of this course, the students; 1) Gain knowledge about the basic principles of biological analysis. 2) Learn isolation and seperation techniques of biomolecules. 3) Discuss the principles involved in undertaking standard sample preparation procedures for analysis of biomolecules. 4) Understand the use of enzymes, antibodies, cells and DNA in biosensors. 5) Gain knowledge about the use of biological molecules in advanced technologies like lab-on-chip and microfluidic analytical systems.
In this course, important analytical methods for characterization of biomolecules such as proteins, DNA, RNA, carbohydrates and lipids are discussed. This course also covers the use of enzymes, antibodies, cells and DNA in analytical systems, e.g. biosensors and emerging technologies like lab-on-chip and microfluidic analytical systems.
COURSE CONTENTS
WEEK
TOPICS
1st Week
Course overview
2nd Week
Sample preparation methods for analysis of proteins and peptides
3rd Week
Protein analysis and detection
4th Week
Enzymatic analysis
5th Week
Antibody-Based Identification Methods
6th Week
Isolation and purification of nucleic acids
7th Week
Analysis of nucleic acids
8th Week
Midterm Exam
9th Week
Separation, purification and analysis of carbohydrates
10th Week
Sample preparation and lipid analysis
11th Week
Bioelectrochemistry and electrochemical biosensors
12th Week
Microfluidic analytical systems
13th Week
Lab-on-chip technology
14th Week
Students Presentations
RECOMENDED OR REQUIRED READING
Bioanalytics: Analytical Methods and Concepts in Biochemistry and Molecular Biology, Friedrich Lottspeich, Joachim W. Engels, Wiley, 2018. Applications of Microfluidic Systems in Biology and Medicine, Manabu Tokeshi, Springer, 2019. Principles and Practice of Bioanalysis, 2nd edition, Richard F. Venn, CRC Press, 2008. Bioanalytical Chemistry, 2nd edition, Andreas Manz, Petra S Dittrich, Dimitri Iossifidis, 2015.
PLANNED LEARNING ACTIVITIES AND TEACHING METHODS
Lecture,Discussion,Questions/Answers,Presentation
ASSESSMENT METHODS AND CRITERIA
Quantity
Percentage(%)
Mid-term
1
40
Presentation of Article
1
20
Total(%)
60
Contribution of In-term Studies to Overall Grade(%)
60
Contribution of Final Examination to Overall Grade(%)
40
Total(%)
100
ECTS WORKLOAD
Activities
Number
Hours
Workload
Midterm exam
Preparation for Quiz
Individual or group work
Preparation for Final exam
1
90
90
Course hours
14
3
42
Preparation for Midterm exam
Laboratory (including preparation)
Final exam
1
3
3
Homework
Presentation (including preperation)
1
70
70
Project
1
95
95
Total Workload
300
Total Workload / 30
10
ECTS Credits of the Course
10
LANGUAGE OF INSTRUCTION
English
WORK PLACEMENT(S)
No
KEY LEARNING OUTCOMES (KLO) / MATRIX OF LEARNING OUTCOMES (LO)