| TYPE OF COURSE UNIT | Compulsory Course |
| LEVEL OF COURSE UNIT | Bachelor's Degree |
| YEAR OF STUDY | 3 |
| SEMESTER | Sixth Term (Spring) |
| NUMBER OF ECTS CREDITS ALLOCATED | 4 |
| NAME OF LECTURER(S) | . Fef Komite
|
| LEARNING OUTCOMES OF THE COURSE UNIT |
At the end of this course, the students;
1) Isolate protein and perform quantitative analysis, visualize on SDS-PAGE, perform Westen blot analysis, and report the experimental results.
2) Perform fundamental chromatographic analysis, and report the experimental results.
3) Determine antioxidant enzyme activity using different techniques and report the experimental results.
4) Determine oxidative stress in cell using different parameters, quantitatively analyze antioxidant molecules of cells, and report the experimental results.
|
| MODE OF DELIVERY | Face to face |
| PRE-REQUISITES OF THE COURSE | No |
| RECOMMENDED OPTIONAL PROGRAMME COMPONENT | None |
| COURSE DEFINITION | This course provides students to learn the basic experimental protocols related to biochemical analysis. |
| COURSE CONTENTS | | WEEK | TOPICS |
|---|
| 1st Week | Introduction | | 2nd Week | Titration | | 3rd Week | Protein isolation and quantitation | | 4th Week | SDS-PAGE | | 5th Week | Western Blotting | | 6th Week | Thin-layer chromatography (TLC) | | 7th Week | Column chromatography | | 8th Week | Midterm Exam | | 9th Week | Zymography (negative activity staining) (SOD) | | 10th Week | Detection of enzyme activity | | 11th Week | Proline assay | | 12th Week | Estimation of lipid peroxidation | | 13th Week | Determination of hydrogen peroxide content | | 14th Week | Determination of DPPH free-radical scavenging activity |
|
| RECOMENDED OR REQUIRED READING | Laboratory manual |
| PLANNED LEARNING ACTIVITIES AND TEACHING METHODS | Experiment,Report Preparation,Lecture |
| ASSESSMENT METHODS AND CRITERIA | | | Quantity | Percentage(%) |
|---|
| Mid-term | 1 | 25 | | Quiz | 1 | 15 | | Practice | 1 | 25 | | Total(%) | | 65 | | Contribution of In-term Studies to Overall Grade(%) | | 65 | | Contribution of Final Examination to Overall Grade(%) | | 35 | | Total(%) | | 100 |
|
| ECTS WORKLOAD |
| Activities |
Number |
Hours |
Workload |
| Midterm exam | 1 | 2 | 2 | | Preparation for Quiz | 12 | ,5 | 6 | | Individual or group work | | | | | Preparation for Final exam | 1 | 7 | 7 | | Course hours | | | | | Preparation for Midterm exam | 1 | 7 | 7 | | Laboratory (including preparation) | 13 | 4 | 52 | | Final exam | 1 | 2 | 2 | | Homework | | | | | Quiz | 12 | ,5 | 6 | | Report writing | 12 | 3,5 | 42 | | Total Workload | | | 124 |
|---|
| Total Workload / 30 | | | 4,13 |
|---|
| ECTS Credits of the Course | | | 4 |
|---|
|
| LANGUAGE OF INSTRUCTION | Turkish |
| WORK PLACEMENT(S) | No |
| | |
