| TYPE OF COURSE UNIT | Compulsory Course |
| LEVEL OF COURSE UNIT | Bachelor's Degree |
| YEAR OF STUDY | 3 |
| SEMESTER | Fifth Term (Fall) |
| NUMBER OF ECTS CREDITS ALLOCATED | 8 |
| NAME OF LECTURER(S) | Assistant Professor Aliye Ezgi Güleç Taşkıran
|
| LEARNING OUTCOMES OF THE COURSE UNIT |
At the end of this course, the students;
1) Apply the steps of molecular cloning.
2) Know how to detect gene transferred by molecular cloning, determine expression level and protein expression.
3) Know how to do experimental studies with the obtained recombinant organism.
4) Knows expression analysis with real-time quantitative polymerase chain reaction (PCR) (qRT-PCR) and interprets the results.
|
| MODE OF DELIVERY | Face to face |
| PRE-REQUISITES OF THE COURSE | No |
| RECOMMENDED OPTIONAL PROGRAMME COMPONENT | None |
| COURSE DEFINITION | In this course, it is aimed to learn the basic principles of molecular cloning, the screening of clones, and the basic experimental protocols for target gene expression analysis and to gain practical knowledge about these techniques. |
| COURSE CONTENTS | | WEEK | TOPICS |
|---|
| 1st Week | Course Description and Primer Design for Target Gene Amplification | | 2nd Week | Competent Cell Preparation | | 3rd Week | Amplification of the Target (PCR) | | 4th Week | Plasmid Isolation (Vector Extraction) | | 5th Week | Cutting Genes and Plasmids | | 6th Week | Ligation | | 7th Week | Transformation | | 8th Week | Midterm Exam | | 9th Week | Screening of Clones (Colony PCR) | | 10th Week | RNA Isolation from Columns | | 11th Week | cDNA Synthesis and RT-PCR | | 12th Week | qRT-PCR | | 13th Week | Functional Analysis in Colonies (Detection of the Level of Circadian Rhythm Genes) | | 14th Week | Repeat and Evaluate |
|
| RECOMENDED OR REQUIRED READING | Gene Cloning and DNA Analysis: An Introduction. T.A. Brown. Sixth/Seventh Edition.
Molecular Biotechnology, Principles and Applications of Recombinant DNA. Bernard R. Glick, Jack J. Pasternak, ASM Press, Washington, D.C., 2009.
|
| PLANNED LEARNING ACTIVITIES AND TEACHING METHODS | Lecture,Experiment,Report Preparation,Other |
| ASSESSMENT METHODS AND CRITERIA | | | Quantity | Percentage(%) |
|---|
| Mid-term | 1 | 25 | | Assignment | 1 | 5 | | Quiz | 10 | 10 | | Practice | 7 | 15 | | Total(%) | | 55 | | Contribution of In-term Studies to Overall Grade(%) | | 55 | | Contribution of Final Examination to Overall Grade(%) | | 45 | | Total(%) | | 100 |
|
| ECTS WORKLOAD |
| Activities |
Number |
Hours |
Workload |
| Midterm exam | 1 | 2 | 2 | | Preparation for Quiz | 11 | 1 | 11 | | Individual or group work | 14 | 3 | 42 | | Preparation for Final exam | 1 | 25 | 25 | | Course hours | 11 | 1 | 11 | | Preparation for Midterm exam | 1 | 20 | 20 | | Laboratory (including preparation) | 13 | 4 | 52 | | Final exam | 1 | 2 | 2 | | Homework | 1 | 26 | 26 | | Quiz | 11 | ,25 | 2,75 | | Report writing | 4 | 12 | 48 | | Total Workload | | | 241,75 |
|---|
| Total Workload / 30 | | | 8,05 |
|---|
| ECTS Credits of the Course | | | 8 |
|---|
|
| LANGUAGE OF INSTRUCTION | Turkish |
| WORK PLACEMENT(S) | No |
| | |
