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COURSE UNIT TITLECOURSE UNIT CODESEMESTERTHEORY + PRACTICE (Hour)ECTS
MOLECULAR BIOLOGY LABORATORY MBG223 Third Term (Fall) 0 + 4 4

TYPE OF COURSE UNITCompulsory Course
LEVEL OF COURSE UNITBachelor's Degree
YEAR OF STUDY2
SEMESTERThird Term (Fall)
NUMBER OF ECTS CREDITS ALLOCATED4
NAME OF LECTURER(S)Associate Professor Ceyhun Kayıhan
. Fef Komite
LEARNING OUTCOMES OF THE COURSE UNIT At the end of this course, the students;
1) apply isolation methods for DNA, RNA and plasmid from the cell, and quantify these isolates, evaltuate the purity of samples.
2) apply basic genetic studies using Drosophila melanogaster, explain the basic terms related to patters of inheritance.
3) amplifies a gene by PCR method and knows, evaluates, and reports other uses of PCR in life and health sciences.
4) determine, analyze and discuss the expression level of a gene.
MODE OF DELIVERYFace to face
PRE-REQUISITES OF THE COURSENo
RECOMMENDED OPTIONAL PROGRAMME COMPONENTNone
COURSE DEFINITIONThis course aim to enable students with learning of techniques such as DNA, RNA, plasmid DNA isolation, RT RT, gel electropheresis used in molecular biology, and practical information about these techniques.
COURSE CONTENTS
WEEKTOPICS
1st Week Introduction
2nd Week DNA isolation (from blood)
3rd Week MiniPrep plasmid isolation
4th Week Spectrophotometric Measurment for Nucleic Acid Quantitation
5th Week Agarose Gel Electrophoresis for Nucleic Acid Quantitation
6th Week PCR
7th Week RAPD
8th Week Midterm Exam
9th Week Restriction Enzyme Digestion
10th Week RFLP
11th Week RNA Isolation
12th Week RT-PCR
13th Week Patterns of Inheritance
14th Week
15th Week
RECOMENDED OR REQUIRED READINGLaboratory manual
PLANNED LEARNING ACTIVITIES AND TEACHING METHODSExperiment,Report Preparation,Lecture
ASSESSMENT METHODS AND CRITERIA
 QuantityPercentage(%)
Mid-term130
Quiz710
Other720
Total(%)60
Contribution of In-term Studies to Overall Grade(%)60
Contribution of Final Examination to Overall Grade(%)40
Total(%)100
ECTS WORKLOAD
Activities Number Hours Workload
Midterm exam122
Preparation for Quiz7214
Individual or group work
Preparation for Final exam11414
Course hours
Preparation for Midterm exam11212
Laboratory (including preparation)11444
Final exam122
Homework
Quiz7,251,75
Report writing7535
Total Workload124,75
Total Workload / 304,15
ECTS Credits of the Course4
LANGUAGE OF INSTRUCTIONTurkish
WORK PLACEMENT(S)No
  

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